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generation lentiviral vectors on retronectin  (TaKaRa)


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    Structured Review

    TaKaRa generation lentiviral vectors on retronectin
    (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to <t>lentiviral</t> transduction on <t>RetroNectin</t> coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.
    Generation Lentiviral Vectors On Retronectin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+lentiviral+vectors+on+retronectin/Culture+Plate/bio_rxiv__2021__09__10__458880-170-14-19
    Average 96 stars, based on 473 article reviews
    generation lentiviral vectors on retronectin - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Role of mitotic diffusion barriers in regulating the asymmetric division of activated CD8 T cells"

    Article Title: Role of mitotic diffusion barriers in regulating the asymmetric division of activated CD8 T cells

    Journal: bioRxiv

    doi: 10.1101/2021.09.10.458880

    (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to lentiviral transduction on RetroNectin coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.
    Figure Legend Snippet: (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to lentiviral transduction on RetroNectin coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.

    Techniques Used: Cell Culture, Transduction, Activation Assay, Expressing, Staining, Imaging, Microscopy

    Related Articles

    Isolation:

    Article Title: Role of mitotic diffusion barriers in regulating the asymmetric division of activated CD8 T cells
    Article Snippet: T cells were cultured at 37° C / 5% CO 2 in enriched RPMI medium containing HEPES (25mM; Sigma-Aldrich), 2-Mercaptoethanol (50μM; Sigma-Aldrich), Sodium Pyruvate (1mM; Life Technologies), MEM Non-essential amino acid solution (1X; Sigma-Aldrich), 10% Fetal Bovine Serum (Thermo Fischer Scientific), antibiotics (penicillin-streptomycin; Invitrogen), and the cytokines IL-7 and IL-15 (10ng/ml and 5ng/ml; PeproTech). .. Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol) for 2d without washing. ..

    Cell Culture:

    Article Title: Role of mitotic diffusion barriers in regulating the asymmetric division of activated CD8 T cells
    Article Snippet: T cells were cultured at 37° C / 5% CO 2 in enriched RPMI medium containing HEPES (25mM; Sigma-Aldrich), 2-Mercaptoethanol (50μM; Sigma-Aldrich), Sodium Pyruvate (1mM; Life Technologies), MEM Non-essential amino acid solution (1X; Sigma-Aldrich), 10% Fetal Bovine Serum (Thermo Fischer Scientific), antibiotics (penicillin-streptomycin; Invitrogen), and the cytokines IL-7 and IL-15 (10ng/ml and 5ng/ml; PeproTech). .. Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol) for 2d without washing. ..



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    generation lentiviral vectors on retronectin  (TaKaRa)
    96
    TaKaRa generation lentiviral vectors on retronectin
    (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to <t>lentiviral</t> transduction on <t>RetroNectin</t> coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.
    Generation Lentiviral Vectors On Retronectin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+lentiviral+vectors+on+retronectin/Culture+Plate/bio_rxiv__2021__09__10__458880-170-14-19
    Average 96 stars, based on 1 article reviews
    generation lentiviral vectors on retronectin - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to lentiviral transduction on RetroNectin coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.

    Journal: bioRxiv

    Article Title: Role of mitotic diffusion barriers in regulating the asymmetric division of activated CD8 T cells

    doi: 10.1101/2021.09.10.458880

    Figure Lengend Snippet: (A) Experimental outline. Murine naïve CD8 T cells, sorted from adult mouse spleen and lymph nodes, were cultured under homeostatic proliferation condition (IL7 and IL15) and subjected to lentiviral transduction on RetroNectin coated cell culture plates, followed by activation or phenotypic analysis. (B) Representative images of CD8 T cells expressing Sec61α-GFP (MemER-GFP) that stained with for CD8. (C and D) Anaphase to telophase transition time of activated CD8 T cells in the imaging medium compared to thermo-reversible hydrogel. Differential interference contrast (DIC) microscopy of dividing CD8 T cells (C) and quantification of anaphase to telophase transitions of dividing CD8 T cells in hydrogel (blue, n=15) or imaging medium (purple, n=18) (unpaired Welch’s t-test; mean ± SEM) (D) . (E) FACS analysis of phenotypic markers (CD62L, CD127, CD44, CD25 and CD69) of lentivirally transduced (purple) or non-transduced CD8 T cells (black) prior to activation (blue), or 48 hrs after TCR-mediated activation (pink). Data are representative of three independent experiments. Unpaired Welch’s t-test; mean ± SEM. Scale bars, 10 μm.

    Article Snippet: Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol) for 2d without washing.

    Techniques: Cell Culture, Transduction, Activation Assay, Expressing, Staining, Imaging, Microscopy